Manisha Nautiyal, Ph.D.
Postdoctoral Research Fellow
Education:
2008 Ph.D.- Molecular Medicine/ Biochemistry, Wake Forest University Health Sciences
1999 M.S.- Organic Chemistry, H.N.B. Garhwal University, Uttaranchal, India
1997 B.S.- Biology, H.N.B. Garhwal University, Uttaranchal, India
Laboratory Department: Hypertension and Vascular Research Center
Email: mnautiya@wfubmc.edu
Advisor: Dr. Debra Diz
Current Research:
My research proposal seeks to evaluate the hypothesis that Ang-(1-7) regulates expression of MAP kinase phosphatase MKP-1 (negative regulator of MAPK) and protein tyrosine phosphatase 1b PTP1b (negative regulator of PI3K) in response to Ang II stimulated activation of the MAPK and PI3K pathways. Transgenic animals with altered brain RAS [(mRen2)27, ASrAOGEN and control Sprague Dawley rats] will be used to dissect out the signaling mechanism utilized by Ang-(1-7), MKP-1 and PTP1b to modulate baroreflex sensitivity and mitochondrial function. The gene and protein expression for downstream components of PI3K and MAPK pathway will be examined and immunolocalization of MKP-1 in brain with respect to Angiotensin peptides will also be investigated. These studies will provide insight into the underlying mechanisms by which brain RAS components can regulate signaling pathways that are altered with aging and under various pathological conditions and will lead to new strategies that can be used to prevent and/or treat hypertension, thereby reducing the morbidity and mortality associated with cardiovascular diseases.
Publications:
Nautiyal M, Islam M, Wynn RM, Mobley JA, Chuang DT and Hutson SM. Identification and characterization of BCAA metabolon proteins: Role of NIPSNAP1 and glutamate dehydrogenase in BCAA metabolism. [Submitted to JBC]
Nautiyal M, Sweatt AJ, MacKenzie JA, Payne RM, Wilkinson JC, Szucs S, Matalon R, Wallin R and Hutson SM. Neuronal localization of the mitochondrial protein NIPSNAP1 in rat brain and nervous system. [In Preparation]
Abstracts:
Nautiyal M, Sweatt AJ, and Hutson SM. “Expression of NIPSNAP1 in Rat Brain: A Comparative Study with the Branched-Chain Amino Acid (BCAA) Metabolizing Enzymes.” J. Neurochem., 2008: 104 (Suppl.1; 1-59). American Society for Neurochemistry (ASN), San Antonio, Texas, Poster Presentation.
Nautiyal M, Islam M, Cavelier L and Hutson SM. “Biophysical Characterization of the Mitochondrial Protein NIPSNAP1.” The FASEB Journal. 2008; 22: 1088.13. Experimental Biology (EB), San Diego, California, Poster Presentation.
Islam M, Nautiyal M, Mobley J and Hutson SM. “Identification and Characterization of Branched-Chain Amino Acid (BCAA) Metabolon Proteins: Role of Glutamate Dehydrogenase” The FASEB Journal. 2008; 22: 610.4. Experimental Biology (EB), San Diego, California, Poster Presentation.
Nautiyal M, Sweatt AJ, and Hutson SM. “Neuronal Localization of the Branched-Chain Amino Acid (BCAA) Metabolon Protein Component, NIPSNAP1, in rat brain.” The 21st Biennial meeting of the International Society for Neurochemistry (ISN). ASN_ISN 2007 Aug 19-24, Cancun, Mexico. Abstract no. 10450. Accepted for poster presentation.
Nautiyal M, Sweatt AJ, and Hutson SM. “Neuronal Localization of the Branched-Chain Amino Acid (BCAA) Metabolon Protein Component, NIPSNAP1, in rat brain.” Western NC Chapter of the Society for Neuroscience (WNCSN). Dec 3, 2007. Wake Forest University School of Medicine, NC. Poster Presentation.
Nautiyal M, Wajih N, Fujii H, Wallin R, Hutson S. “ Identification of NIPSNAP1 as a component of a supramolecular complex of leucine catabolic enzymes.” The FASEB Journal. 2004 March 24;18 (5): A848. Experimental Biology-2004, Washington DC, Oral presentation.
Honors and Awards:
2009-2010: Warren Trust Postdoctoral Cardiovascular Fellowship Sponsored by The Consortium for Southeastern Hypertension Control.
2008: Wake Forest University Graduate School of Arts and Sciences
Alumni Student Travel Award
2006-2008: American Heart Association Pre-doctoral Fellowship
2005-2006: Molecular Medicine (NIH-T32) Training Grant