Peter A. Antinozzi

Associate Professor, Biochemistry

Email: pantinoz@wfubmc.edu

Education:

Assistant Professor of Biochemistry and Internal Medicine (Section of Molecular Medicine) Additional Affiliations: Diabetes Center; Graduate Faculty (Biochemistry, Molecular Medicine, Molecular Genetics)

B.S. - Chemistry and Microbiology (1985-1989) University of Florida, Gainesville, FL
Ph.D. - Cell and Molecular Biology (1992-1999) University of Texas, Southwestern, Dallas, TX
Research Fellow - Clinical Biochemistry (1999-2002) University of Geneva, Geneva, Switzerland
Research Fellow - Cellular Biophysics (2002-2004) Memorial Sloan-Kettering Cancer Center, New York, NY
Research Faculty - Physiology and Cellular Biophysics (2004-2007) Columbia University, New York, NY

Research Interests:

Functional genomics. Advances in high-throughput genotyping technologies and large collaborative efforts which combine genetic datasets have brought to light several novel candidate genes involved in a variety of diseases. In particular, studies of complex trait diseases such as diabetes and cardiovascular disease have been great beneficiaries of these efforts and a number of highly replicated loci have been identified. The bottleneck in translating these findings towards clinical intervention is determining the causative mechanisms of the allelic variants within these loci. To this end, one of the primary goals of my laboratory is to leverage clinically relevant genetic data with functional data at the cellular level.

Functional mapping. One of our current initiatives is "functional mapping of diabetes-susceptibility loci" to identify novel genes involved in diabetes. In brief, we systematically dissect clinically relevant chromosome regions with molecular biology strategies that alter the expression of candidate genes. Expression of specific allelic variants and/or gene knockdown (via RNA interference methods) are functionally assessed by a panel of cell-based functional assays.



High-throughput, high-content screening. A second area of research in my laboratory is the development of cell-based assays for our functional mapping projects. The goal of this research area is to increase the throughput of the functional screening by assay miniturization and multiplexing readouts. Our current strategies include a combination of assays that are image-based (via automated fluorescence microscopy) and plate reader-based (fluorescence, luminescence, absorbance).

Recent Publications:

Antinozzi PA, Garcia-Diaz A, Hu C, Rothman JE. Functional mapping of disease susceptibility loci using cell biology. Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3698-703. Epub 2006 Feb 28.

Wang H, Iezzi M, Theander S, Antinozzi PA, Gauthier BR, Halban PA, Wollheim CB. Suppression of Pdx-1 perturbs proinsulin processing, insulin secretion and GLP-1 signalling in INS-1 cells. Diabetologia. 2005 Apr;48(4):720-31. Epub 2005 Mar 9.

Wang H, Maechler P, Antinozzi PA, Herrero L, Hagenfeldt-Johansson KA, Bjorklund A, Wollheim CB. The transcription factor SREBP-1c is instrumental in the development of beta-cell dysfunction. J Biol Chem. 2003 May 9;278(19):16622-9

Rubi B, Antinozzi PA, Herrero L, Ishihara H, Asins G, Serra D, Wollheim CB, Maechler P, Hegardt FG. Adenovirus-mediated overexpression of liver carnitine palmitoyltransferase I in INS1E cells: effects on cell metabolism and insulin secretion. Biochem J. 2002 May 15;364(Pt 1):219-26.

Antinozzi PA, Ishihara H, Newgard CB, Wollheim CB. Mitochondrial metabolism sets the maximal limit of fuel-stimulated insulin secretion in a model pancreatic beta cell: a survey of four fuel secretagogues. J Biol Chem. 2002 Apr 5;277(14):11746-55. Epub 2002 Jan 30.

Publications:
For a listing of additional publications, refer to PubMed, a service provided by the National Library of Medicine