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Anatomic Pathology Laboratory Services

MICROMED:

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MICROMED is a full service microscopy laboratory supplying equipment and technical support for both electron and confocal microscopy; it comprises a shared resource available throughout the institution for both clinical and research purposes.

The EM equipment includes conventional transmission and scanning electron microscopy (TEM and SEM). The facility also houses a laser scanning confocal microscope (LSCM), digital upright and inverted fluorescence microscopes, time-lapse microscopy, laser capture microdissection, and light amplification fluorescence and luminescence systems for non-invasive imaging in living rodents. The LSCM is capable of multiphoton excitation.

This resource exists in a biomedical research environment and supports numerous investigators whose interests encompass both basic and clinical medical science. The facility is considered a core laboratory under the Comprehensive Cancer Center grant, providing additional support for microscopy to investigators affiliated with the Cancer Center.

Although the orientation is biomedical, the facility supports a wide range of research. Faculty and staff also have extensive experience in non-biomedical zoology, botany, and materials science imaging.

Additionally, the laboratory offers numerous specialized research techniques, user training, consultation, and collaboration. MICROMED is equipped to carry out all phases of sample preparation for electron and confocal microscopy with a staff that includes a technical engineer/manager and two technologists.

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TELEPHONE NUMBERS:

PCR Lab: (336) 716-2670

STAFF:

Director: Mark Willingham, MD
Tel: (336) 716-7779
Fax: (336) 716-7595
mwilling@wfubmc.edu

Supervisor and Chief EM Technologist: Ken Grant
Tel: (336) 716-6175
kgrant@wfubmc.edu

EM Technologists:
Bilinda Dawson
Tel: (336) 716-2670
bdawson@wfubmc.edu
Paula Moore:
Tel: (336) 716-2670
pmoore@wfubmc.edu

Admin. Secretary:
Mary Ann Van Dusen

Tel: (336) 716-2675
mavandus@wfubmc.edu

 

 

 

Capabilities:

  • TEM (120 keV)
  • SEM (5-30 keV)
  • LSCM with multiphoton excitation
  • Bright field, DIC, phase contrast , polarizing, fluorescence and video light microscopy
  • Immunolabeling for LM, EM, SEM and LSCM
  • Autoradiography and enzyme cytochemistry
  • Cyromicrotomy, CryoIVEM, and EDX compositional microanalysis
  • Image processing and analysis
  • Laser capture microdissection
  • Single cell microinjection
  • Tumor and cell imaging using GFP or luciferase reporters in living rodents

Instrumentation:

  • Philips 400 120 keV TEM with high tilt goniometer stage
  • Philips 515 SEM with backscatter, cathodoluminescence, and EDAX detectors
  • Zeiss LSM510 laser scanning confocal system
  • Arcturus PixCell II laser microdissection system
  • Zeiss Axioplan 2 and Olympus IX-70 fluorescence microscopes
  • Zeiss Axiovert time-lapse video microscopy system for cultured cells
  • Reichert Ultracut microtomes and cryoultramicrotomes
  • Computer workstations with image processing, analysis, and 3-D reconstruction software
  • Photographic darkrooms
  • Complete specimen preparation facilities

 

 

 

 

 

Copyright: Wake Forest University School of Medicine and North Carolina Baptist Hospitals. All rights reserved.

Medical Center Boulevard

Winston-Salem, NC 27157

The information on this Website is for general informational purposes only and SHOULD NOT be relied upon as a substitute for sound professional medical advice, evaluation or care from your physician or other qualified healthcare provider. If you have a medical problem or a health-related question, consult your physician or call Health On-Call at 336-716-2255 or 1-800-446-2255.

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Last Modified: 3/27/2008