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Intravital Microscopy

Intravital Microscopy   Clip # Run      time Aspect of intravital microscopy shown 1 0:00-0:20 Phosphate buffered saline infusion 2 0:21-0:30 Fluorescent labeling of the vasculature 3 0:30-0:53 Spread of fluorescent label in the microvasculature 4 0:53-1:04 Stage micrometer 5 1:05-1:18 Phosphate buffered saline infusion 6 1:19-1:56 Real-time visualization of fluorescent labeled cells injected intraarterially 7 1:57-2:45 Visualization of fluorescent labeled cells in a vessel, both with and without the fluorescent filter   Clip # Significance 1 Shows resolution in vessels of various sizes, and the contrast between normal blood flow and PBS injection 2 Shows ability to label and detect labeling of vasculature 3 Shows ability to label and detect labeling of the microvasculature 4 A means to measure vessel diameters, and distances that rolling cells travel on vascular endothelium 5 Same as clip one; different preparation 6 Shows feasibility of detection of labeled cells 7 When fluorescent filter is on, single labeled cell is detectable in the circulation (2:11 and another at 2:12); when fluorescent filter is off, interaction of cells with vascular endothelium can be observed