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Polymerase Chain Reaction (PCR)-based technology allows detection of DNA or RNA that may be specific for certain microorganisms, genes, or gene products. Currently the PCR lab offers the following:
- BCR-abl
- Cytomegalovirus
- Enterovirus
- Epstein Barr virus
- Herpes simplex virus
- Herpes varicella/zoster virus
- Herpes VIII (KSHV)
- Human papilloma virus
- Immunoglobulin heavy chain
- Toxoplasma
All specimens submitted for analysis must be accompanied by a requistion form containing the following information:
- Patient name,
- ordering physician and address,
- date of collection,
- test required, and
- specimen source.
Samples for PCR may be submitted in several ways:
- Paraffin embedded tissue on glass slides
- Paraffin block
- Air-dried or Tzanck smear on glass slide
- Fresh or frozen tissue. The use of formalin is discouraged
- Fresh body fluid. (blood, vitreous, CSF) Blood should be collected in EDTA, NOT HEPARIN. There must be a separate tube collected for PCR tests. Tubes that have been opened and sampled for other tests will be rejected.
Normal turnaround time is estimated to be 3-5 days depending upon the day the sample is received.
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TELEPHONE NUMBER:
(336) 716-9880
STAFF:
Director, Molecular
Diagnostics and PCR Labs:
Mark Willingham, MD
Tel: (336) 716-7779
Fax: (336) 716-7595
mwilling@wfubmc.edu
Manager:
Lamar Jones Tel: (336) 716-2644 lajones@wfubmc.edu
Assistant Manager:
Martha Ward
Tel: (336) 716-2756
mward@wfubmc.edu
LAB HANDBOOK:
Medical Center staff can click here to use the online Laboratory Handbook (only available on in-house computers connected to the infiNet). The handbook contains detailed information about all tests performed, in-house as well as send-outs, and instructions for specimen collection.
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